Perez lab
Center for Human Immunology Lausanne
Laboratory of molecular immunology and vaccinology
Principal Investigator: Professor Laurent Perez
email: [email protected]
Laboratory of molecular immunology and vaccinology
Principal Investigator: Professor Laurent Perez
email: [email protected]
Group members
Alison Lin : PhD Student
Pauline Nortier : PhD Student
Rachel Schelling : Research Associate
David Vinals Sales : PhD Student
Pauline Nortier : PhD Student
Rachel Schelling : Research Associate
David Vinals Sales : PhD Student
Research Focus
Our laboratory focuses on the analysis and characterization of the humoral immune response in human. Our main goal is to understand the molecular basis underlying B cells response in healthy and pathological conditions.
Specifically, we aim to:
Our group is highly multidisciplinary and use immunological, cellular, biochemical and structural approaches in combination with next generation sequencing and single cell transcriptomic methodologies.
Specifically, we aim to:
- Understand the molecular interaction between antigen and antibody using human antibody cloning, antibody-based vaccine design and structural biology.
- Improve antibodies response to vaccine using nanoparticles and novel scaffold designed by computer-aided design.
- Investigate the abnormal B-cells response in pathological setting of autoimmunity.
- Follow the antibody repertoire response in case of chronic infections.
Our group is highly multidisciplinary and use immunological, cellular, biochemical and structural approaches in combination with next generation sequencing and single cell transcriptomic methodologies.
Current projects Covers
- Identification of molecular switches for B lymphocyte activation or inhibition.
- Mechanisms of HCMV mediated immune evasion.
- Structural elucidation of antibodies mediating neutralization of SARS-CoV-2.
- Computer-aided design of nanoparticles for next generation vaccine.
- Cross neutralization of human Pneumoviridae.
Selected Publications
Characterizing cytomegalovirus infection one cell at a time. Perez L, 2021. Nature microbiology, 6 (12) pp. 1477-1478.
https://www.nature.com/articles/s41564-021-00994-w
Fenwick, C*, Turelli, P*, Perez, L*, Pellaton, C, Esteves-Leuenberger, L., Farina, A., Campos, J., Lana, E., Fiscalini, F., Raclot, C., Pojer, F, Lau, K, Demurtas, D, Descatoire, M, Joo, VS, Foglierini, M,Noto, A, Abdelnabi, R, Foo, C, Vangeel, J N, Du,w, Bosch,J, Veldman,G, Leyssen,P, Thiel,V, LeGrand,R Lévy,Y, Trono,D, Pantaleo, D. 2021. A highly potent antibody effective against SARS-CoV-2 variants of concern. Cell Report. *: equal contribution
https://www.sciencedirect.com/science/article/pii/S221112472101278X
Perotti M, Marcandalli J, Demurtas D, Sallusto F and Perez L. Rationally designed Human Cytomegalovirus gB nanoparticle vaccine with improved immunogenicity. PLoS Pathogens. 2020 16(12) : e1009169. [10.1371/journal.ppat.1009169] https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009169
Foglierini M, Pappas L, Lanzavecchia A, Corti D, and Perez L. AncesTree: An interactive immunoglobulin lineage tree visualizer. PLoS Comput Biol. 2020 16(7): e1007731. [10.1371/journal.pcbi.1007731].
https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1007731
Marcandalli J, Fiala B, Ols S, Perotti M, Snijder J, Hodge E, Lanzavecchia A, Sallusto F, Dubois P, Lee K, Veesler D, Stewart L, Baker D, Lore K, Perez L*, King NP*. Induction of potent neutralizing antibody responses by a designed protein nanoparticle RSV vaccine. Cell, 2019. Mar 7;176 (6):1420-1431.e17. doi: 10.1016/j.cell.2019.01.046. *: equal contribution and corresponding authors
https://www.sciencedirect.com/science/article/pii/S0092867419301096?via%3Dihub
Martinez-Martin N, Marcandalli J, Huang CS, Arthur CP, Perotti M, Foglierini M, Ho H, Dosey AM, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L*, Ciferri C*. An unbiased screen for Human Cytomegalovirus identifies Neuropilin-2 as a central viral receptor. Cell, 2018. Aug 23;174 (5):1158- 1171.e19. doi: 10.1016/j.cell.2018.06.028.
*: equal contribution and corresponding authors.
https://www.sciencedirect.com/science/article/pii/S0092867418307967?via%3Dihub
Kabanova A, Marcandalli J, Zhou T, Bianchi S, Baxa U, Tsybovsky Y, Lilleri D, Silacci-Fregni C, Foglierini M, Fernandez-Rodriguez BM, Druz A, Zhang B, Geiger R, Pagani M, Sallusto F, Kwong PD, Corti D, Lanzavecchia A and Perez L. Platelet-derived growth factor-alpha receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer. Nature Microbiology, 2016. Jun 6;1(8):16082. doi: 10.1038/nmicrobiol.2016.82. https://pubmed.ncbi.nlm.nih.gov/27573107/
https://www.nature.com/articles/s41564-021-00994-w
Fenwick, C*, Turelli, P*, Perez, L*, Pellaton, C, Esteves-Leuenberger, L., Farina, A., Campos, J., Lana, E., Fiscalini, F., Raclot, C., Pojer, F, Lau, K, Demurtas, D, Descatoire, M, Joo, VS, Foglierini, M,Noto, A, Abdelnabi, R, Foo, C, Vangeel, J N, Du,w, Bosch,J, Veldman,G, Leyssen,P, Thiel,V, LeGrand,R Lévy,Y, Trono,D, Pantaleo, D. 2021. A highly potent antibody effective against SARS-CoV-2 variants of concern. Cell Report. *: equal contribution
https://www.sciencedirect.com/science/article/pii/S221112472101278X
Perotti M, Marcandalli J, Demurtas D, Sallusto F and Perez L. Rationally designed Human Cytomegalovirus gB nanoparticle vaccine with improved immunogenicity. PLoS Pathogens. 2020 16(12) : e1009169. [10.1371/journal.ppat.1009169] https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009169
Foglierini M, Pappas L, Lanzavecchia A, Corti D, and Perez L. AncesTree: An interactive immunoglobulin lineage tree visualizer. PLoS Comput Biol. 2020 16(7): e1007731. [10.1371/journal.pcbi.1007731].
https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1007731
Marcandalli J, Fiala B, Ols S, Perotti M, Snijder J, Hodge E, Lanzavecchia A, Sallusto F, Dubois P, Lee K, Veesler D, Stewart L, Baker D, Lore K, Perez L*, King NP*. Induction of potent neutralizing antibody responses by a designed protein nanoparticle RSV vaccine. Cell, 2019. Mar 7;176 (6):1420-1431.e17. doi: 10.1016/j.cell.2019.01.046. *: equal contribution and corresponding authors
https://www.sciencedirect.com/science/article/pii/S0092867419301096?via%3Dihub
Martinez-Martin N, Marcandalli J, Huang CS, Arthur CP, Perotti M, Foglierini M, Ho H, Dosey AM, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L*, Ciferri C*. An unbiased screen for Human Cytomegalovirus identifies Neuropilin-2 as a central viral receptor. Cell, 2018. Aug 23;174 (5):1158- 1171.e19. doi: 10.1016/j.cell.2018.06.028.
*: equal contribution and corresponding authors.
https://www.sciencedirect.com/science/article/pii/S0092867418307967?via%3Dihub
Kabanova A, Marcandalli J, Zhou T, Bianchi S, Baxa U, Tsybovsky Y, Lilleri D, Silacci-Fregni C, Foglierini M, Fernandez-Rodriguez BM, Druz A, Zhang B, Geiger R, Pagani M, Sallusto F, Kwong PD, Corti D, Lanzavecchia A and Perez L. Platelet-derived growth factor-alpha receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer. Nature Microbiology, 2016. Jun 6;1(8):16082. doi: 10.1038/nmicrobiol.2016.82. https://pubmed.ncbi.nlm.nih.gov/27573107/
Funding
